Bahram Falahatkar; Khayam Delafkar; Masoud Sattari; Hossein Khara
Volume 5, Issue 3 , March 2017, , Pages 79-96
Abstract
The present study was carried out to investigate the efficacy of two anesthetics, clove oil and ketamine, comparison of hematological parameters, and blood biochemical indices under optimum doses of the two anesthetics in juvenile Sterlet Acipenser ruthenus (n=276) with an average weight of 64.1 ± ...
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The present study was carried out to investigate the efficacy of two anesthetics, clove oil and ketamine, comparison of hematological parameters, and blood biochemical indices under optimum doses of the two anesthetics in juvenile Sterlet Acipenser ruthenus (n=276) with an average weight of 64.1 ± 3.4 g. Clove oil was used in concentrations of 14, 35, 56, 77, 98 and 119 mg/l, and those of 35, 37.5, 40, 42.5, 45 and 50 mg/l for ketamine in three replicates. Both losing time of equilibrium and induced time of anesthesia were reduced by increasing the concentrations of these two agents, whereas time of recovery increased. Optimum doses regarding the time of anesthesia and recovery were obtained as 56 mg/l and 45 mg/l for clove oil and ketamine, respectively. There were significant decreases in hematological parameters for all treatments compared to control group (P<0.05). No significant changes were observed in cortisol, glucose, and lactate concentrations for fish treated with clove oil (P>0.05), whereas, fish exposed to handling stress showed significant changes in all biochemical indices (P<0.05). On the other hand, glucose concentration did not change significantly in ketamine treatment (P>0.05), but cortisol and lactate changed significantly (P<0.05). Considering appropriate efficacy of clove oil in both induction and recovery times, and rendering no changes in biochemical indices, clove oil would be recommended as an alternative to ketamine for this species during aquaculture practices.
